Nightingale College had a total enrollment of 6,036 students in 2024. The full-time enrollment at Nightingale College is 3,922 students and the part-time enrollment is 2,114. This means that 65% of students enrolled at Nightingale College are enrolled full-time.

The enrolled student population at Nightingale College, both undergraduate and graduate, is 25.1% Hispanic or Latino, 24.3% White, 20.5% Asian, 19.3% Black or African American, 2.2% Native Hawaiian or Other Pacific Islanders, 1.09% American Indian or Alaska Native, and 0.0994% Two or More Races.

Students enrolled at Nightingale College in full-time Undergraduate programs are most commonly White Female (21.5%), followed by Hispanic or Latino Female (20.4%) and Asian Female (16.5%). Students enrolled in full-time Graduate programs are most commonly White Female (48.4%), followed by Hispanic or Latino Female (22.6%) and White Male (11.3%).