Messiah University had a total enrollment of 3,373 students in 2024. The full-time enrollment at Messiah University is 2,486 students and the part-time enrollment is 887. This means that 73.7% of students enrolled at Messiah University are enrolled full-time.

The enrolled student population at Messiah University, both undergraduate and graduate, is 75.2% White, 8.51% Hispanic or Latino, 4.71% Black or African American, 4% Two or More Races, 3.68% Asian, 0.0889% American Indian or Alaska Native, and 0.0889% Native Hawaiian or Other Pacific Islanders.

Students enrolled at Messiah University in full-time Undergraduate programs are most commonly White Female (43.2%), followed by White Male (32.2%) and Hispanic or Latino Female (5.82%). Students enrolled in full-time Graduate programs are most commonly White Female (57.6%), followed by White Male (18.8%) and Hispanic or Latino Female (6.16%).